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Objective To study and analyze the effect of amlodipine besylate and amlodipine besylate in the treatment of hypertension. Methods 100 patients with hypertension treated in our hospital from January 2015 to December 2016 were selected and randomly divided into two groups, the control group and the experimental group, with 50 patients in each group. The control group was treated with amlodipine besylate, and the patients in the experimental group were treated with amlodipine besylate and amlodipine besylate. The therapeutic effects of the experimental group and the control group were compared and analyzed. Results After the corresponding treatment, the experimental group of 50 cases, the total effective number of 44 cases, 6 patients invalid, the total effective rate was 88%. In the control group of 50 cases, the total effective number was 37 cases, 13 cases were invalid, the total effective rate was 74%. The treatment effect of the experimental group was significantly higher than that of the control group, with statistical difference (P<0.05). There were 5 cases of adverse reactions in the experimental group, 3 patients had facial flushing, and 2 patients suffered from pain. The incidence was 10%. The number of adverse reactions in the control group was 12 cases, ankle edema occurred in 5 patients, and headache occurred in 7 patients, with a rate of 24%. The rate of adverse reactions in the control group was significantly higher than that in the experimental group, with statistical difference (P<0.05). Conclusion Amlodipine and Levamlodipine besylate in treatment of hypertension can improve the treatment efficiency to a great extent, high safety, low probability of adverse reactions, with further clinical promotion and application significance.
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Objective To study the promotive effect of neovascularization on rats with cerebral infarction by nasal administration of granulocyte colony-stimulating factor.Methods A blinded,vehicle-controlled study of ING-CSF and IHG-CSF administration was performed by intraluminal middle cerebral artery occlusion (MCAO) model.All Sprague-Dawley rats were randomly divided into sham-operation group,model group,INNS group,IHGCSF group and ING-CSF group.The neurologic behavioral tests were assessed after reperfusion 72 h.Mter 72 h of MCAO,the brains of rats were stainned with TTC and the infarcted volume was calculated by computer image analysis.The expression of vascular endothelial growth factor (VEGF) in the brain was determined by immune-histochemistry.The density of angiogenesis in the brain was counted under fluorescence microscope.Results The score of neurological function of ING-CSF group(3.90± 1.65)was improved significantly compared with the IHG-CSF group (10.55±2.19) at the point of 72 h after cerebral infarction (P＜0.01).The cerebral infarct volume of ING-CSF group((20.01±3.29) ％) was reduced evidently compared with the IHG-CSF group((33.48±4.49) ％) at 72 h (P＜ 0.01);while the cerebral infarct volume of INNS group ((60.20±7.72) ％)was not markedly different compared with the model group((61.49±6.41)％) at 72 h (P＞0.05).The expression of VEGF in the brains of ING-CSF group was significantly higher than other groups at 72 h.Conclusion Intranasal administration G-CSF can improve neurological function and vascular angiogenesis in rats following MCAO.
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Objective To explore the protective effect of rhG-CSF given intranasally on cerebral infarct rats by observing the neurological dysfunction and the expression of Fas ligand (FasL) in hippocampus of cerebral infarct rats.Methods Middle cerebral artery occlusion(MCAO) model rats were established by nylon strand,reperfuse 2 hours later,and give rhG-CSF through subcutaneous and intranasal way.The rats were divided into the nermal group,the sham-operated control group(sham),MCAO group,MCAO+NS given intranasally group(NS),MCAO + rhG-CSF given subcutaneously group,and MCAO + rhG-CSF given intranasally group each group had 6 rats. At the time of 3d after reperfusion,neurological severity scores (NSS) test was performed and the expression of FasL was detected via immunohistochemical staining in collateral hippocampus. Results Neurological dysfunction appeared in all groups except for the normal and the sham group. The dysfunction of the MCAO and the NS group was the most serious,the NSS was the highest(10.20±1.85,10.30±1.76),the number of FasL positive cells was the most(41.17±3.25,41.00±2.76),and there was no obvious difference between the two groups ( P ＞0.05);the NSS and FasL positive cells decreased in the subcutaneous group(5.67±1.32,P ＜0.01;32.67±1.97,P ＜0.01) and decreased further more in the intranasal group(4.00±0.93,P ＜0.05;19.50±1.05,P ＜0.01).Conclusions rhG-CSF given intranasally can relieve the neurological dysfunction of cerebral infarct rats,and brain cells are thereby protected by resisting the expression of FasL.
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Purpose:To study the expression and clinical significance of PTEN in non-small cell lung cancer. Methods:Immunohistochemical staining was used to determine the expression of PTEN in 10 cases of normal lung issues and 56 cases of non-small cell lung cancer. Results:The positive incidences of PTEN expression in NSCLC was 59% while positive expression was found in all normal lung issues. The expression of PTEN was correlated to degree of differention of cancer, lymph node metastasis and clinical stages. The PTEN negative group was seen to have the worst survival rate among all groups. Conclusions:The results suggest that PTEN gene plays an important role in carcinogenesis and progress of NSCLC. Expression of PTEN may be valuable in prognosis assessment of NSCLC.